Immunohistochemical analysis of paraffin-embedded Esophagus. 1, Antibody was diluted at 1:200 (4°,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Tonsil. 1, Antibody was diluted at 1:200 (4°,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min).

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

This antibody detects endogenous levels of Actin, muscle specific.

Store at -20°C. Stable for one year from the date of shipment.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

 IHC-p 1:200-500

(Optimal dilutions should be determined by the end user)