Histone H3(Phospho Thr6) Rabbit pAb

Key facts:

Catalog NO:	TDY228
Source :	Rabbit
Type:	Polyclonal
Application:	WB,IHC
Reactivity:	H, R, M
Isotype:	IgG
MW(KDa):	15-17
Alternative Names:	H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody

Specifications:

Source

A Rabbit Polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Histone H3(Phospho Thr6) Rabbit Polyclonal antibody can detect endogenous Histone H3(Phospho Thr6) proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000 IHC 1:200-500

(Optimal dilutions should be determined by the end user)

Background:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Applications:

Western Blot

Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using Phospho-Histone H3 (Thr6)Rabbit pAb (upper) TDY228 1:1000 and Histone H3 (1G1) Mouse mAb (lower) TDY055 1:2000.

IHC	Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H3(Phospho Thr6)（TDY228）Rabbit pAb diluted at 1:500.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".