Vimentin Mouse mAb(1A7)

Key facts:

Catalog NO:	TDY199
Source :	Mouse
Type:	Monoclonal
Application:	WB, IF, IHC
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	50-57
Alternative Names:	FLJ36605 antibody, VIM antibody, VIME antibody

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing recombinant protein.

Specificity

The Vimentin mouse monoclonal antibody can detect endogenous Vimentin proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500

(Optimal dilutions should be determined by the end user)

Background:

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.

Applications:

Western Blot

Western blot analysis of 1) Hela, 2)Mouse Brain Tissue , 3) Rat Brain tissue with TDY199 diluted at 1:2,000.

IF	IF analysis of Hela with TDY199(Left) and DAPI (Right) diluted at 1:100.

IHC	Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin （TDY199）Mouse mAb diluted at 1:500.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".