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α Skeletal Muslce Actin Mouse mAb(4B11)
Key facts:
Catalog NO:TDY189
Source :Mouse
Type:Monoclonal
Application:WB, IP, IHC
Reactivity:H, R, M
Isotype:IgG
MW(KDa):42
Alternative Names:ACTA antibody, ACTA1 antibody, ACTC antibody, ACTC1 antibody, Actin alpha cardiac muscle antibody, ACTS antibody, Alpha actin 1 antibody, Cardiac muscle alpha actin 1 antibody, Skeletal muscle alpha actin 1 antibody
Specifications:

Source

A Mouse monoclonal antibody is prepared by immunizing recombinant protein.


Specificity

The α Skeletal Muslce Actin mouse monoclonal antibody can recognize endogenous α Skeletal Muslce Actin proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:5,000-10,000 IP 1:200  IHC 1:200-500

(Optimal dilutions should be determined by the end user)

 

 

Background:

Reactivity:    H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig

 

Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus

Applications:

Western Blot

 

Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue with TDY189 diluted at 1:20,000.

IP

 

1Input: Mouse Brain Tissue Lysate
2IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY189                                   1:10,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000
IHC

 

Immunohistochemical analysis of paraffin-embedded  Mouse Skeletal Muscle Tissue using α skeletal muscle actin TDY189Mouse mAb diluted at 1:500.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".