eIF4A1 Mouse mAb(M8)

Key facts:

Catalog NO:	TDY164
Source :	Mouse
Type:	Monoclonal
Application:	WB, IF, IHC
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	48
Alternative Names:	DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The eIF4A1 antibody can recognize endogenous eIF4A1 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.

Applications:

Western Blot

Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with TDY164 diluted at 1:2,000.

IF	IF analysis of Hela with TDY164(Left) and DAPI (Right) diluted at 1:100.

IHC	Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（TDY164）Mouse mAb diluted at 1:200.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".