EGFR Mouse mAb(M6)

Key facts:

Catalog NO:	TDY162
Source :	Mouse
Type:	Monoclonal
Application:	WB, IP, IF, IHC
Reactivity:	H
Isotype:	IgG1
MW(KDa):	170
Alternative Names:	Epidermal growth factor receptor antibody, erbb 1 antibody, Receptor tyrosine protein kinase ErbB 1 antibody

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The EGFR antibody can recognize endogenous EGFR proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-3,000 IP 1:200 IF 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.

Applications:

Western Blot

Western blot analysis of 1) A431 ,2) Hela, with TDY162 diluted at 1:2,000.

IP	1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : TDY162 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific(S003), 1:5,000

IF	IF analysis of Hela with TDY162(Left) and DAPI (Right) diluted at 1:100.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".