Cleaved PARP Mouse mAb(M4)

Key facts:

Catalog NO:	TDY160
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC
Reactivity:	H
Isotype:	IgG1
MW(KDa):	116(Pro),89(Active)
Alternative Names:	PARP-1, Poly(ADP ribose) polymerase 1, sPARP1,ADPRT1, ADP ribosyltransferase NAD(+)

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Cleaved PARP antibody can recognize endogenous pro and active PARP protein.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-3,000 IHC 1:200-500

(Optimal dilutions should be determined by the end user)

Background:

Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD⁺ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Applications:

Western Blot

Western blot analysis of 1) Jurkat ,2) Hela, with TDY160 diluted at 1:2,000.

IHC	Immunohistochemical analysis of paraffin-embedded human Tonsil Tissue using Cleaved PARP （TDY160）Mouse mAb diluted at 1:500.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".