His-Tag Mouse mAb(M1)

Key facts:

Catalog NO:	TDY157
Source :	Mouse
Type:	Monoclonal
Application:	WB, IP
Reactivity:	N/A
Isotype:	IgG1
MW(KDa):	N/A
Alternative Names:	6 His antibody, 6*His

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:3,000-5,000 IP 1:500

(Optimal dilutions should be determined by the end user)

Background:

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Applications:

Western Blot

Western blot analysis of His-Tag recombinant protein with TDY157 diluted at 1:5,000

1) His-Tag transfected 293T

2) His-MBP recombinant protein

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".