α-tubulin Mouse mAb(8F11)

Catalog NO:	TDY048
Source :	Mouse
Type:	Monoclonal
Application:	WB, IP, IF ,IHC
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	52
Alternative Names:	alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain

Source

Mouse monoclonal antibody is prepared by immunizing recombinant protein.

Specificity

The α-tubulin mouse monoclonal antibody can detect endogenous α-tubulin proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500

(Optimal dilutions should be determined by the end user)

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin.

Western Blot

Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with TDY048 diluted at 1:5,000.

IHC	Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using α tubulin （TDY048）Mouse mAb diluted at 1:500.

IP	1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : TDY048 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000

IF	IF analysis of Hela with TDY048(Left) and DAPI (Right) diluted at 1:100.

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".