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Histone H3 (tri methyl K79) Mouse mAb(3G3)
Key facts:
Catalog NO:TDY118
Source :Mouse
Type:Monoclonal
Application:WB,IHC,IP
Reactivity:H, R, M
Isotype:IgG1
MW(KDa):15
Alternative Names:H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Histone H3 (tri methyl K79) antibody can recognize endogenous Histone H3 (tri methyl K79) proteins.

 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.


Dilutions

WB 1:500-2,000 IP 1:200  IHC 1:200-500

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. 

 

Applications:

Western Blot

 

Western blot analysis of Hela with Histone H3 (tri methyl K79) Mouse mAb(3G3) diluted at

1）1:2,000

2）1:5,000


Western Blot

 

Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 with Histone H3 (tri methyl K79) (3G3) Mouse MAb diluted at 1:2000.

IP

 

1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY118   1:1,000
Secondary antibody: Goat anti-Mouse IgG(H+L) (S001)     1:10,000

 

IHC

 

Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using  Histone H3(tri methyl K79)（TDY118）Mouse mAb diluted at 1:500.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".