Caspase 9 Mouse mAb(TDY3-20)

Catalog NO:	TDY102
Source :	Mouse
Type:	Monoclonal
Application:	WB, IP, IF
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	47KD(H), 51KD(R, M)
Alternative Names:	APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody

Source

Mouse monoclonal antibody is prepared by immunizing recombinant protein.

Specificity

The Caspase 9 antibody can recognize endogenous Caspase 9 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-5,000 IP 1:200 IF 1:100-200

(Optimal dilutions should be determined by the end user)

Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Western Blot

Western blot analysis of Hela with Caspase 9 (TDY3-20) Mouse mAb diluted at

1）1:2,000

2）1:5,000

Western Blot

Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (TDY3-20) Mouse mAb diluted at 1:3,000

IP	1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : TDY102 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000

IF	IF analysis of Hela with TDY102(Left) and DAPI (Right) diluted at 1:100.

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".