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FH/Fumarase Mouse mAb(7F1)
Key facts:
Catalog NO:TDY098
Source :Mouse
Type:Monoclonal
Application:WB, IF
Reactivity:H, R, M
Isotype:IgG1
MW(KDa):48
Alternative Names:Fumarate hydratase mitochondrial antibody, MCL antibody, MCUL 1 antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The FH mouse monoclonal antibody can detect endogenous FH proteins.

 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.


Dilutions

WB 1:3,000 IF 1:100-200

(Optimal dilutions should be determined by the end user)

 

 

Background:

Reactivity:    H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig

 

Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. 

 

Applications:

Western Blot

 

Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, with FH Mouse mAb diluted at 1:3,000.


Western Blot

 

Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, with FH Mouse mAb diluted at 1:3,000.


 
IF

 

IF analysis of Hela with TDY098 diluted at 1:100.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".