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NFκB p65 Mouse mAb(14H2)
Key facts:
Catalog NO:TDY076
Source :Mouse
Type:Monoclonal
Application:WB, IHC, IP
Reactivity:H,M,R
Isotype:IgG1
MW(KDa):65
Alternative Names:Nuclear Factor NF Kappa B p65 Subunit antibody, p65 NF kappaB antibody, Transcription Factor p65 antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The NFκB p65 mouse monoclonal antibody can detect endogenous NFκB p65 proteins.

 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.


Dilutions

WB 1:1,000-3,000 IHC 1:200 IP 1:200

(Optimal dilutions should be determined by the end user)

 

 

Background:

Reactivity:    H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig

 

NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection. 

 

Applications:

Western Blot

 

Western blot analysis of 1) Hela, 2)293T,3)HepG with NFκB p65 mAb diluted at 1:2,000.

Immunohistochemistry

 

IHC staining of mouse hippocampus tissue with NFκB p65 mouse mAb(14H2) diluted at 1:200.


 
Western Blot

  

Western blot analysis of 1) Mouse Heart, 2)Rat Heart,3)Rat Liver, 4) Rat Spleen with NFκB p65 mAb diluted at 1:3,000.

IP

 

1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : TDY076     1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".