p53 Mouse mAb(6C4)

Key facts:

Catalog NO:	TDY075
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC, IF
Reactivity:	H
Isotype:	IgG1
MW(KDa):	53
Alternative Names:	Cellular tumor antigen p53 antibody, Tumor protein p53 antibody, TRP53 antibody, Antigen NY-CO-13 antibody

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The p53 mouse monoclonal antibody can detect endogenous p53 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:2,000 IHC 1:200 IF 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

Applications:

Western Blot

Western blot analysis of 293T, with p53 mAb diluted at

1) 1:1,000

2) 1:2,000

Immunohistochemistry

IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb（6C4）diluted at 1:200.

IF	IF analysis of Hela with TDY075(Left) and DAPI (Right) diluted at 1:100.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".