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HSP70 Mouse mAb(3G10)
Key facts:
Catalog NO:TDY062
Source :Mouse
Type:Monoclonal
Application:WB, IF, IHC
Reactivity:H, M, R,Pg
Isotype:IgG1
MW(KDa):70
Alternative Names:heat shock protein 70 antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.


Specificity

The HSP70 mouse monoclonal antibody can detect endogenous HSP70 proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:1,000-2,000 IF 1:100-200  IHC 1:200-500

(Optimal dilutions should be determined by the end user)

 

 

Background:

 

The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis. 

 

Applications:

Western Blot

 

Western blot analysis of 1) Hela, 2) Mouse Brain, with HSP70 mAb diluted at 1:2,000.



Western Blot

 

Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2,000.

 

IF

 

IF analysis of Hela with TDY062(Left) and DAPI (Right) diluted at 1:100.

IHC

 

Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using HSP70 TDY062Mouse mAb diluted at 1:500.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".