Bcl-2 Mouse mAb(6B5)

Key facts:

Catalog NO:	TDY061
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC, IF
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	26
Alternative Names:	Apoptosis regulator Bcl 2 antibody, Bcl 2 antibody, B cell leukemia/lymphoma 2 antibody

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Bcl-2 mouse monoclonal antibody can detect endogenous Bcl-2 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-2,000 IHC 1:200 IF 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability.

Applications:

Western Blot

Western blot analysis of Hela with Bcl-2 mouse mAb(6B5) diluted at 1:1,000.

Immunohistochemistry

IHC staining of Human tonsil tissue paraffin-embedded with Bcl-2 mouse mAb（6B5）diluted at 1:200.

Western Blot

Western blot analysis of 3T3 with Bcl-2 mouse mAb(6B5) diluted at 1:1,000.

IF	IF analysis of Hela with TDY061(Left) and DAPI (Right) diluted at 1:100.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".