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T7-Tag Mouse mAb(1H5)
Key facts:
Catalog NO:TDY012
Source :Mouse
Type:Monoclonal
Application:WB
Reactivity:N/A
Isotype:IgG1
MW(KDa):N/A
Alternative Names:Major capsid protein 10A antibody, MASMTGGQQMG epitope tag antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.


Specificity

The T7 tag antibody can recognize T7-tag fusion proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:5,000

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein. 
Applications:

Western Blot

 

0.5ug T7 fusion protein+ Primary antibody dilution at

11:3,000

21:5,000

31:10,000

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".