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MBP-Tag Mouse mAb(7G2)
Key facts:
Catalog NO:TDY008
Source :Mouse
Type:Monoclonal
Application:WB
Reactivity:N/A
Isotype:IgG1
MW(KDa):N/A
Alternative Names:MBP antibody, Maltose Binding Protein antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing MBP protein.


Specificity

The MBP tag antibody can recognize MBP and MBP-tag fusion proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:5,000

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. 
Applications:

Western Blot

 

0.5ug MBP fusion protein+ Primary antibody dilution at

11:3,000

21:5,000

31:10,000

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".