Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

Store at -20°C. Stable for one year from the date of shipment.

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

WB 1:5,000 IP 1:200 IF 1:1,000

(Optimal dilutions should be determined by the end user)

IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with His-tag fusion protein

3、IP (untransfected 293+anti-His mAb+ Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-His mAb+ Protein G agarose)

6、Recombinant protein (E.coli)

IF analysis of 293 cells transfected with a His-tag protein, using TDYbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His)

2ug His fusion protein+ Primary antibody dilution at

1、1:5,000

2、1:10,000

1、Multi-Tag Recombinant protein

2、293T transfected with His recombinant protein

3、Insect cell trandfected with His recombinant protein