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Flag-Tag Mouse mAb(2C5)
Key facts:
Catalog NO:TDY001
Source :Mouse
Type:Monoclonal
Application:WB, IP, IF
Reactivity:N/A
Isotype:IgG1
MW(KDa):N/A
Alternative Names:DDDDK Tag antibody, DYKDDDDK Tag antibody
Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.


Dilutions

WB 1:5,000 IP 1:200 IF 1:2,000

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. 
Applications:

IP

 

IP antibody use：5ug Flag Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with Flag-tag fusion protein

3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

4、IP (transfected 293+anti- Flag mAb+ Protein G agarose)

5、IP (transfected 293+Protein G)

6、Recombinant protein (E.coli)



Western Blot

 

1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.

 

IF

 

IF analysis of 293 cells transfected with a Flag-tag protein, using TDYbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".