β-tubulin Mouse mAb(5G3)

Key facts:

Catalog NO:	TDY043
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC, IF
Reactivity:	H, R, M, Mk, Dg, Ch, Hm, Rb, Sh, Pg, Insect, Yeast
Isotype:	IgG1
MW(KDa):	55
Alternative Names:	beta tubulin antibody, TUBB antibody

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The β-tubulin mouse monoclonal antibody can detect endogenous β-tubulin proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:5,000 IHC 1:200 IF 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Applications:

Western Blot

Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.

Immunohistochemistry

IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.

IF	IF analysis of Hela with TDY043(Left) and DAPI (Right) diluted at 1:100.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".