β-actin Mouse mAb(5B7)

Key facts:

Catalog NO:	TDY041
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC, IF
Reactivity:	H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh, Insect
Isotype:	IgG1
MW(KDa):	43
Alternative Names:	actin antibody, b-actin antibody, beta-actin antibody

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The β-actin mouse monoclonal antibody can detect endogenous β-actin proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:5,000 IHC 1:200 IF 1:200

(Optimal dilutions should be determined by the end user)

Background:

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Applications:

Western Blot

Western blot analysis of Hela (1) Rat brain (2)Mouse brain (3) Chicken lung (4) and Rabbit testis (5)with β-Actin mouse mAb(5B7) diluted at 1:10,000.

Immunohistochemistry

IHC Staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200

Western Blot

Western blot analysis of Hamster Kidney with β-Actin mouse mAb(5B7) diluted at 1:10,000.

Western Blot

Western blot analysis of 293T(1) , NIH3T3(2) with β-Actin mouse mAb(5B7) diluted at 1:10,000.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".