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Trk B (Phospho Tyr706) Rabbit pAb
Key facts:
Catalog NO:TP0270
Source :Rabbit
Type:Polyclonal
Application:WB,IF,ELISA,IHC
Reactivity:H,M,R
Isotype:IgG
MW(KDa):145kd or 92kd(with limited samples)
Alternative Names:NTRK2 ; TRKB ; BDNF/NT-3 growth factors receptor ; GP145-TrkB ; Trk-B ; Neurotrophic tyrosine kinase receptor type 2 ; TrkB tyrosine kinase ; Tropomyosin-related kinase B
Specifications:
Source The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr705. AA range:671-720

 

Specificity Phospho-Trk B (Y706) Polyclonal Antibody detects endogenous levels of Trk B protein only when phosphorylated at Y706.The name of modified sites may be influenced by many factors

 

Storage Store at -20°C. Stable for one year.

 

Concentration 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

 

Dilutions

WB 1:500-2,000; IHC 1:100-300; IF 1:200-1000; ELISA 1:20,000 

(Optimal dilutions should be determined by the end user)
Background:
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that , upon neurotrophin binding , phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants.
Applications:
Western Blot                                        

Western Blot analysis of 293 cells using Phospho-Trk B (Y706) Polyclonal Antibody diluted at 1:1000
Western Blot                                                           

Western blot analysis of lysates from mouse kidney, using Trk B (Phospho-Tyr705) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.
IF                                                                                                                                                                                                                                                                                                                                                                                                                                     

Immunofluorescence analysis of Hela cell. 1,Trk B (phospho Tyr706) Polyclonal Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 28.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".