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p21 (9Z10) Rabbit Monoclonal Antibody
Key facts:
Catalog NO:TR8508
Source :Rabbit
Type:Monoclonal
Application:WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
Reactivity:Mouse,Rat
Isotype:IgG, Kappa
MW(KDa):18kDa
Alternative Names:Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;CAP20;CDKN1;CIP1;MDA6;PIC1;SDI1;WAF1;
Specifications:
Source A Rabbit Monoclonal antibody is prepared by immunizing recombinant protein.

 

Specificity Endogenous

 

Storage Store at -20°C. Stable for one year.

 

Concentration 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

 

Dilutions

  IHC-P 1:500-1:2000, WB 1:2000-1:10000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200

(Optimal dilutions should be determined by the end user)

Background:
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes , and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53 , through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen , a DNA polymerase accessory factor , and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases , which thus leads to a dramatic activation of cyclin-dependent kinase2 , and may be instrumental in the execution of apoptosis following caspase activation.
Applications:
Western  Blot                            

Rat womb cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 28.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".