Beclin 1 Rabbit Monoclonal Antibody

Key facts:

Catalog NO:	TR8000
Source :	Rabbit
Type:	Monoclonal
Application:	WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
Reactivity:	Human,Mouse,Rat
Isotype:	IgG, Kappa
MW(KDa):	60kDa
Alternative Names:	Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197);GT197

Specifications:

Specificity

Endogenous

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

IHC-P 1:200-1:1000, WB 1:2000-1:10000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200

(Optimal dilutions should be determined by the end user)

Background:

beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

Applications:

Western Blot

Western blot analysis of lysates from Hela cells, using Beclin 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4℃,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".