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NF-κB p65 (Acetyl Lys310) Rabbit mAb
Key facts:
Catalog NO:TR9209
Source :Rabbit
Type:Monoclonal
Application:WB,IF,IP,ELISA
Reactivity:H,M
Isotype:IgG,Kappa
MW(KDa):60(calculated),65(Observed)
Alternative Names:RELA ; NFKB3 ; Transcription factor p65 ; Nuclear factor NF-kappa-B p65 subunit ; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Specifications:
Source

A Rabbit Monoclonal antibody is prepared by immunizing recombinant protein.

 

Specificity

NF-κB p65 (Acetyl Lys310) Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at K310.

 

Storage

 Store at -20°C. Stable for one year from the date of shipment.

 

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

 

Dilutions

WB 1:2,000-10,000; IF 1:200-1,000; IP 1:50-200; ELISA 1:5,000-20,000

(Optimal dilutions should be determined by the end user)
   
Background:
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Applications:
Western  Blot                                                                                                                                                                                                                                                                                        

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-κB p65 (Acetyl Lys310) (PT1367R) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: NIH-3T3 Predicted band size: 60kDa Observed band size: 65kDa
 
Immunohistochemistry

 


Immuno?uorescence analysis of Hela cell. 1,NFκB-p65 (Acetyl Lys310) Antibody(red) was diluted at 1:200. β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200
Western Blot                                                                                                                                                                                                                                                                                                                                 


Immunofluorescence analysis of Mouse-testis tissue. 1,NFκB-p65 (AcetylLys310) Antibody(red) was diluted at 1:200. 2, Cy3 labledSecondary antibody was diluted at 1:300.3,Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C:merge of A+B
Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".