Flag-Tag Rabbit pAb

Key facts:

Catalog NO:	TDY1112
Source :	Rabbit
Type:	Polyclonal
Application:	WB
Reactivity:	Species independent
Isotype:	IgG
MW(KDa):
Alternative Names:	DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Antibody can detects recombinant Flag-Tag proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB: 1:5,000-20,000

(Optimal dilutions should be determined by the end user)

Background:

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Applications:

Western Blot

Western blot analysis of 293T Transfected 1) 293T, 2)pEGFP-C1, 3) 3*Flag-EGFP, 4) EGFP-3*Flag Cell Lysate using Flag-Tag (TDY1112）Rabbit Polyclonal Antibody diluted at 1:5,000.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".