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PI 3 kinase p85/p55 (Phospho Tyr467/199) Rabbit pAb
Key facts:
Catalog NO:TDYP0224
Source :Rabbit
Type:Polyclonal
Application:WB,IHC,IF,ELISA
Reactivity:H,M,R,Mk，Pg
Isotype:IgG
MW(KDa):55,85
Alternative Names:PI3KR1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha ; PI3-kinase regulatory subunit alpha ; PI3K regulatory subunit alpha ; PtdIns-3-kinase regulatory subunit alpha ; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha ; PI3-kinase subunit p85- alpha ; PtdIns-3-kinase regulatory subunit p85-alpha
Specifications:
Source

The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199. AA range:436-485

 

Specificity

This antibody detects endogenous levels of PI3 kinase p85/p55

 

Storage

Store at -20°C. Stable for one year from the date of shipment.

 

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

 

Dilutions

WB 1:500-2,000; IHC 1:100-300; IF 1:200-1,000; ELISA 1:10,000

(Optimal dilutions should be determined by the end user)
Background:
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
Applications:
Western Blot                                 

                        

Western Blot analysis of various cells using primary antibody diluted at 1:1000

 
Immunohistochemistry

           

Immunohistochemical analysis of para?n-embedded Human-lung tissue. 1,PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200

 
Western Blot                                                        

                

Western blot analysis of lysates from COS7 cells using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody

 
Immunofluorescence            

          

Immunofluorescence analysis of rat-spleen tissue. 1,PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".