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Histone H3 Rabbit pAb
Key facts:
Catalog NO:TDY1004
Source :Rabbit
Type:Polyclonal
Application:WB, IP
Reactivity:C. Elegans,H,M,R,Yeast
Isotype:IgG
MW(KDa):17
Alternative Names:H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Specifications:

Source

Rabbit Polyclonal  antibody is prepared by immunizing Recombinant Protein.

Specificity

The  Histone H3 Rabbit Polyclonal  Antibody can detects endogenous  Histone H3  proteins.

 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.


Dilutions

WB 1:2,000 -5,000   IP 5uL/mg protein 

(Optimal dilutions should be determined by the end user)

 

 

Background:

 

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Applications:

 

WB

 

 

 

 

Western blot analysis of Lysate using （TDY1004）

 

Rabbit pAb diluted at 1:4,000.
WB

 

 

Western blot analysis of 1) Yeast Lysate, 2) Drosophila Melanogaster Lysate, 3)C Elegans Lysate,4）Raw Cell Lysate, 5）Rat Brain Tissue Lysate using Histone H3 （TDY1004）Rabbit pAb diluted at 1:5,000.
IP

 

 

IP: Western blot analysis of 1) Hela Cell Lystate INPUT, 2) Hela Cell Lysate Histone H3 Rabbit pAb , 3)Hela Cell Lysate Rabbit Control IgG, using Histone H3 （TDY1004）Rabbit pAb diluted at 1:5,000.

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".