Claudin-2 Mouse mAb(8H11)

Key facts:

Catalog NO:	TDY766
Source :	Mouse
Type:	Monoclonal
Application:	WB,IHC
Reactivity:	H,R,M
Isotype:	IgG1
MW(KDa):	25
Alternative Names:	Claudin2,CLDN2,SP82

Specifications:

Source

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Claudin-2 mouse monoclonal antibody can detect endogenous Claudin-2 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-2,000 IHC:1;100-1:200

(Optimal dilutions should be determined by the end user)

Background:

The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer . Claudin-2 is expressed primarily in the proximal tubule of the normal mammalian kidney, where it regulates transepithelial ion (e.g., Na+, Cl-) reabsorption . Increased expression of Claudin-2 has been reported in some cancer cell lines , including A549 lung adenocarcinoma cells, where its nuclear distribution was positively associated with enhanced proliferation .

Applications:

Western Blot

Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Heart Tissue Lysate , 3) Rat Heart Tissue Lysate using Claudin-2 （TDY766）Mouse mAb diluted at 1:2000.

IHC

Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma Tissue using Claudin-2 （TDY766）Mouse mAb diluted at 1:200.

IHC

Immunohistochemical analysis of paraffin-embedded Human Endometrial Carcinoma Tissue using Claudin-2 （TDY766）Mouse mAb diluted at 1:200.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".