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ATG5 Mouse mAb(3C7)
Key facts:
Catalog NO:TDY720
Source :Mouse
Type:Monoclonal
Application:WB,IHC
Reactivity:H,R,M
Isotype:IgG1
MW(KDa):55
Alternative Names:APG5, APG5L, ASP, Autophagy protein 5 antibody
Specifications:

Source

Mouse Monoclonal antibody is prepared by immunizing Recombinant Protein.

Specificity

The  ATG5  Mouse Monoclonal  Antibody can detects endogenous  ATG5  proteins.

 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.


Dilutions

WB  1:5,000-10,000

IHC 1:100-200

(Optimal dilutions should be determined by the end user)

 

 

Background:

Reactivity:    H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig

 

Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Applications:

 

IHC

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 （TDY720）Mouse mAb diluted at 1:200.

IHC

 

 

  

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 （TDY720）Mouse mAb diluted at 1:200. 
WB

 

 Western blot analysis of Hela Cell Lysate using ATG5 （TDY720）Mouse mAb diluted at 1:10000.

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".