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P44/42 MAPK(ERK1/2) Mouse mAb(1H4)
Key facts:
Catalog NO:TDY645
Source :Mouse
Type:Monoclonal
Application:WB, IHC
Reactivity:H, R, M
Isotype:IgG1
MW(KDa):44/42
Alternative Names:MAPK, Mitogen-activated protein Kinase, PRKM2,MK01
Specifications:

Source

Mouse Monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.


Specificity

The P44/42 MAPK(ERK1/2) Mouse Monoclonal antibody can recognize endogenous P44/42 MAPK(ERK1/2) proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB  1:1,000-2,000      IHC 1:100-200

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer.
Applications:

 

Western Blot

 

 

 

 
 

Western blot analysis of 1) Hela Cell Lysate, 2)Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using ERK1/2TDY645Mouse mAb diluted at 1:2000.

IHC

 

  

 
Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using ERK1/2TDY645Mouse mAb diluted at 1:200.
 
IHC

 

  

 
Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using ERK1/2TDY645Mouse mAb diluted at 1:200.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".