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LC3A Mouse mAb(5G10)
Key facts:
Catalog NO:TDY617
Source :Mouse
Type:Monoclonal
Application:WB,IHC
Reactivity:H, R, M
Isotype:IgG1
MW(KDa):14,16
Alternative Names:ATG8E, MAP1LC3A, autophage-related protein LC3A
Specifications:

Source

Mouse Monoclonal antibody is prepared by immunizing Recombinant Protein.


Specificity

The LC3A Mouse Monoclonal antibody can recognize endogenous LC3A proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

 WB  1:1,000-2,000     IHC 1:100-200

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

 

Applications:
 
Western Blot

 

 

Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using LC3A TDY617 Mouse mAb diluted at 1:1000.

 

IHC

 

  

 

Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3ATDY617Mouse mAb diluted at 1:200.

IHC

  

 

 

Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A TDY617 Mouse mAb diluted at 1:200.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".