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Akt(Phospho Ser473) Mouse mAb(7F9)
Key facts:
Catalog NO:TDY589
Source :Mouse
Type:Monoclonal
Application:WB, IHC
Reactivity:H
Isotype:IgG1
MW(KDa):60
Alternative Names:c Akt antibody, MGC9965 antibody, Protein kinase B antibody, RAC antibody, PRKBA antibody
Specifications:

Source

Mouse Monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.


Specificity

The Akt(Phospho Ser473) Mouse Monoclonal antibody can recognize endogenous Akt1 proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:1,000-2,000    IHC 1:100-200

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
Akt, is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. 
 

 

Applications:

Western Blot

 

 

 

 

Western blot analysis of PC3 Cell Lysateusing Phospho-Akt Ser473TDY589Mouse mAb diluted at 1:1000.

 

IHC

 

 

 

Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473TDY589Mouse mAb diluted at 1:200.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".