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AMPK α1 Mouse mAb(5G11)
Key facts:
Catalog NO:TDY495
Source :Mouse
Type:Monoclonal
Application:WB,IHC
Reactivity:H, R, M
Isotype:IgG1
MW(KDa):63
Alternative Names:AAPK1, 5 AMP activated protein kinase alpha 1 catalytic subunit, PRKAA1, SNF1A, MGC33776
Specifications:

Source

A Mouse Monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.


Specificity

The AMPK α1 Mouse Monoclonal antibody can recognize endogenous AMPK α1 proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:1,000-2,000    IHC 1:100-200

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3).

 

Applications:

Western Blot

 

Western blot analysis of 1)Hela, 2) 293T, 3)3T3, 4) PC12 with AMPK a1 Mouse mAb TDY495 diluted at 1:2,000.

IHC

 

  

Immunohistochemical analysis of paraffin-embedded Mouse ColonTissue using AMPK a1 (TDY495) Mouse mAb diluted at 1:200.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".