Smad3 Mouse mAb(8B5)

Key facts:

Catalog NO:	TDY481
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	50
Alternative Names:	hMAD3 antibody, HSPC193 antibody, LDS1C antibody, MADH3 antibody, JV15 2 antibody

Specifications:

Source

A Mouse Monoclonal antibody is prepared by immunizing recombinant protein.

Specificity

The Smad3 Mouse Monoclonal antibody can recognize endogenous Smad3 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-2,000 IHC 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription.

Applications:

Western Blot

Western blot analysis of 1)293T, 2)Jurkat, 3)Rat Heart Tissue, 4)Mouse Heart Tissue with Smad3 Mouse mAb TDY481 diluted at 1:2,000.

IHC	Immunohistochemical analysis of paraffin-embedded Human Liver Tissue using Smad3（TDY481）Mouse mAb diluted at 1:200.

IHC	Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using Smad3（TDY481）Mouse mAb diluted at 1:200.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".