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GAPDH Rabbit pAb(G04-For Bacterium)
Key facts:
Catalog NO:TDY421
Source :Rabbit
Type:Polyclonal
Application:WB, IHC
Reactivity:H, R, M, Bacterium
Isotype:IgG
MW(KDa):37
Alternative Names:G3PDH antibody, BARS-38 antibody, Glycerasldehyde 3 phosphate dehydrogenase antibody
Specifications:

Source

A Rabbit Polyclonal antibody is prepared by immunizing recombinant protein.


Specificity

The GAPDH Rabbit Polycloan antibody can recognize endogenous GAPDH proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:5,000-10,000   IHC  1:200-500

(Optimal dilutions should be determined by the end user)

 

 

Background:
 
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Applications:

Western Blot

 

Western blot analysis of Hela with GAPDH Rabbit pAb TDY421 diluted at 1:10,000.

IHC

 

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using  GAPDH (TDY421Rabbit pAb diluted at 1:500.

 

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".