Active Caspase 3 Mouse mAb(5E1)

Key facts:

Catalog NO:	TDY407
Source :	Mouse
Type:	Monoclonal
Application:	WB, IHC
Reactivity:	H, R, M
Isotype:	IgG1
MW(KDa):	17
Alternative Names:	APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody

Specifications:

Source

A Mouse Monoclonal antibody is prepared by immunizing recombinant protein.

Specificity

The Active Caspase 3 Mouse Monoclonal antibody can recognize endogenous Active Caspase 3 proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:500-1,000 IHC 1:100-200

(Optimal dilutions should be determined by the end user)

Background:

Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.

Applications:

Western Blot

Western blot analysis of 1) Hela, 2)3T3, 3)Rat Brain Tissue with Active Caspase 3 Mouse mAb TDY407 diluted at 1:1,000.

IHC	Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase 3 （TDY407）Mouse mAb diluted at 1:200.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".