Histone H2A.X(Acetyl Lys5) Rabbit pAb

Key facts:

Catalog NO:	TDY284
Source :	Rabbit
Type:	Polyclonal
Application:	WB, IHC
Reactivity:	H, R, M
Isotype:	Antiserum
MW(KDa):	15
Alternative Names:	H2A histone family member X antidoby, H2AFX antibody, H2AX Histoneh antibody, Histone H2AX antibody

Specifications:

Source

A Rabbit Polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Specificity

The Histone H2A.X(Acetyl Lys5) Rabbit Polyclonal antibody can detect endogenous Histone H2A.X(Acetyl Lys5) proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Concentration

Rabbit Antiserum, containing 0.02% sodium azide and 50% glycerol.

Dilutions

WB 1:1,000-2,000 IHC 1:200-500

(Optimal dilutions should be determined by the end user)

Background:

Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Applications:

Western Blot

Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using Acetyl-Histone H2A.X (Lys5) Rabbit pAb (upper) TDY284 1:2,000 and β-actin (5B7) Mouse mAb (lower) TDY041 1:5,000.

IHC	Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H2A.X (Acetyl Lys5)（TDY284）Rabbit pAb diluted at 1:500.

Contact information:

Company:	BEI JING TDY BIOTECH CO.,LTD.
Orders:	TDYbiotech@163.com
Website:	http://www.tdybio.com
Telephone:	010-80117836
Address:	Zhen Xin Road 36.Changping Distict,Beijing

Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig

Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation

Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".