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Histone H2A.X(Phospho Tyr142) Rabbit pAb
Key facts:
Catalog NO:TDY282
Source :Rabbit
Type:Polyclonal
Application:WB, IHC
Reactivity:H, R, M
Isotype:IgG
MW(KDa):15
Alternative Names:H2A histone family member X antidoby, H2AFX antibody, H2AX Histoneh antibody, Histone H2AX antibody
Specifications:

Source

A Rabbit Polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.


Specificity

The Histone H2A.X(Phospho Tyr142) Rabbit Polyclonal antibody can detect endogenous Histone H2A.X(Phospho Tyr142) proteins.


 
Storage

Store at -20°C. Stable for one year from the date of shipment.


 
Concentration

Immunogen Affinity Purified Rabbit IgG,1mg/ml in PBS pH 7.4, containing 0.02% sodium azide and 50% glycerol.



Dilutions

WB 1:1,000-2,000   IHC  1;200-500

(Optimal dilutions should be determined by the end user)

 

 

Background:

 

Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. 

 

Applications:

Western Blot

 

Western blot analysis of extracts from Hela cells, using Phospho-Histone H2A.X(Tyr142) Rabbit pAb (upper) TDY282 1:2,000.

IHC

 

Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using  Histone H2A.X (Phospho Tyr142)TDY282Rabbit pAb diluted at 1:500.

Contact information:
Company:BEI JING TDY BIOTECH CO.,LTD.
Orders:TDYbiotech@163.com
Website:http://www.tdybio.com
Telephone:010-80117836
Address:Zhen Xin Road 36.Changping Distict,Beijing
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Please note: All products are"FOR RESEARCH USE ONLY.NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES".