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| Flag-Tag Mouse mAb(2C5)-HRP conjugated |
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| DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag |
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| Cat#： TDY034 | Source#： Mouse | Type#： Monoclonal | |
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| Applications: WB |
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Reactivity: N/A |
| MW.(KDa): N/A |
Isotype: IgG1 |
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The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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| Western Blot |
Western blot analysis of Recombinant protein with TDY034 diluted at
1) 1:5,000
2) 1:10,000
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. Antibody conjugated to HRP.
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| Specificity |
The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% Kathon and 50% glycerol.
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| Dilutions |
WB 1:5,000-10,000
(Optimal dilutions should be determined by the end user)
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DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag |
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| Size: |
TDY034C-50μL ￥2000
TDY034F-100μL ￥3500 |
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| New Product: |
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| 直接下单: |
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立即下单
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