| Cat#： TDY032 | Source#： Mouse | Type#： Monoclonal | |
 |
| Applications: WB |
|
Reactivity: N/A |
| MW.(KDa): N/A |
Isotype: IgG1 |
|
|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
|
|
|
|
| Western Blot |
Western blot analysis of His recombinant protein with His Mouse mAb(4E6) HRP Conjugated diluted at
1）1:10,000
2）1:20,000
|
|
|
| |
|
|
| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH. Antibody conjugated to HRP.
|
| Specificity |
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
|
| Storage |
Store at -20°C. Stable for one year from the date of shipment.
|
| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% Kathon and 50% glycerol.
|
| Dilutions |
WB 1:3,000-10,000
(Optimal dilutions should be determined by the end user)
|
|
|
| |
|
|
|
|
| Size: |
TDY032C-50μL ￥2000
TDY032F-100μL ￥3500 |
|
| New Product: |
|
|
|
| 直接下单: |
|
立即下单
|
|
