| Cat#： TDY024 | Source#： Rabbit | Type#： Polyclonal | |
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| Applications: WB |
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Reactivity: N/A |
| MW.(KDa): N/A |
Isotype: IgG |
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A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
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| Western Blot |
1ug His fusion protein+ Primary antibody dilution at
1、1:5,000
2、1:10,000
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| Western Blot |
Primary antibody dilution at 1:5,000
1、293T transfeted with His tag recombinant protein
2、Baculovirus Expression Vector System transfected with His tag recombinant protein
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| Source |
Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:5,000
(Optimal dilutions should be determined by the end user)
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| Size: |
TDY024C-50μL ￥800
TDY024F-100μL ￥1500 |
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| New Product: |
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| 直接下单: |
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立即下单
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