IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with V5-tag fusion protein

3、IP(untransfted 293+anti-V5 mAb+Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-V5 mAb+ Protein G agarose)

6、IP (transfected 293+Protein G)

7、Recombinant protein (E.coli)

IF analysis of 293 cells transfected with a V5-tag protein, using TDYbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)

1ug V5 fusion protein+ Primary antibody dilution at

1、1:5,000

2、1:10,000

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

The V5 tag antibody can recognize C-terminal, internal, and N-terminal V5-tag fusion proteins.

Store at -20°C. Stable for one year from the date of shipment.

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

WB 1:5,000 IP 1:200 IF 1:1,000

(Optimal dilutions should be determined by the end user)