| Cat#： TR8508 | Source#： Rabbit | Type#： Monoclonal | |
 |
| Applications: WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA |
|
Reactivity: Mouse,Rat |
| MW.(KDa): 18kDa |
Isotype: IgG, Kappa |
|
| This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes , and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53 , through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen , a DNA polymerase accessory factor , and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases , which thus leads to a dramatic activation of cyclin-dependent kinase2 , and may be instrumental in the execution of apoptosis following caspase activation. |
|
|
| Western Blot |
Rat womb cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
|
|
| |
|
| Source |
A Rabbit Monoclonal antibody is prepared by immunizing recombinant protein. |
| Storage |
Store at -20°C. Stable for one year. |
| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
| Dilutions |
IHC-P 1:500-1:2000, WB 1:2000-1:10000, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200
(Optimal dilutions should be determined by the end user)
|
|
| |
|
| Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;CAP20;CDKN1;CIP1;MDA6;PIC1;SDI1;WAF1; |
| |
|
|
|
|
| Size: |
50μL ￥1300
100μL ￥2300 |
|
| New Product: |
|
|
|
| 直接下单: |
|
立即下单
|
|
