| Cat#： TR9209 | Source#： Rabbit | Type#： Monoclonal | |
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| Applications: WB,IF,IP,ELISA |
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Reactivity: H,M |
| MW.(KDa): 60(calculated),65(Observed) |
Isotype: IgG,Kappa |
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| NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011], |
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| Western Blot |
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-κB p65 (Acetyl Lys310) (PT1367R) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: NIH-3T3 Predicted band size: 60kDa Observed band size: 65kDa
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| Immunohistochemistry |
Immuno?uorescence analysis of Hela cell. 1,NFκB-p65 (Acetyl Lys310) Antibody(red) was diluted at 1:200. β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200
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| Western Blot |
Immunofluorescence analysis of Mouse-testis tissue. 1,NFκB-p65 (AcetylLys310) Antibody(red) was diluted at 1:200. 2, Cy3 labledSecondary antibody was diluted at 1:300.3,Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C:merge of A+B
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| Source |
A Rabbit Monoclonal antibody is prepared by immunizing recombinant protein.
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| Specificity |
NF-κB p65 (Acetyl Lys310) Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at K310.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:2,000-10,000; IF 1:200-1,000; IP 1:50-200; ELISA 1:5,000-20,000
(Optimal dilutions should be determined by the end user)
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| RELA ; NFKB3 ; Transcription factor p65 ; Nuclear factor NF-kappa-B p65 subunit ; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
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| Size: |
50μL ￥1300
100μL ￥2300 |
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| New Product: |
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| 直接下单: |
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立即下单
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