| Cat#： TDY1112 | Source#： Rabbit | Type#： Polyclonal | |
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| Applications: WB |
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Reactivity: Species independent |
| MW.(KDa): |
Isotype: IgG |
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| The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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| Western Blot |
Western blot analysis of 293T Transfected 1) 293T, 2)pEGFP-C1, 3) 3*Flag-EGFP, 4) EGFP-3*Flag Cell Lysate using Flag-Tag (TDY1112）Rabbit Polyclonal Antibody diluted at 1:5,000.
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The Antibody can detects recombinant Flag-Tag proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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Dilutions |
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WB: 1:5,000-20,000
(Optimal dilutions should be determined by the end user)
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| DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag |
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| Size: |
50μL ￥800
100μL ￥1500 |
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| New Product: |
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| 直接下单: |
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立即下单
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