| Cat#: TDYP0224 | Source#: Rabbit | Type#: Polyclonal | |
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| Applications: WB,IHC,IF,ELISA |
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Reactivity: H,M,R,Mk,Pg |
| MW.(KDa): 55,85 |
Isotype: IgG |
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| Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The
enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four
transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
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| Western Blot |

Western Blot analysis of various cells using primary antibody diluted at 1:1000
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| Immunohistochemistry |

Immunohistochemical analysis of para?n-embedded Human-lung tissue. 1,PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200
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| Western Blot |

Western blot analysis of lysates from COS7 cells using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody
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| Immunofluorescence |

Immunofluorescence analysis of rat-spleen tissue. 1,PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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| Source |
The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199. AA range:436-485
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| Specificity |
This antibody detects endogenous levels of PI3 kinase p85/p55
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:500-2,000; IHC 1:100-300; IF 1:200-1,000; ELISA 1:10,000
(Optimal dilutions should be determined by the end user)
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| PI3KR1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha ; PI3-kinase regulatory
subunit alpha ; PI3K regulatory subunit alpha ; PtdIns-3-kinase regulatory subunit alpha ;
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha ; PI3-kinase subunit p85-
alpha ; PtdIns-3-kinase regulatory subunit p85-alpha
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| Size: |
50μL ¥1000 100μL ¥1800 |
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| New Product: |
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